北京油鸡肌肉和卵巢组织基因组DNA甲基化状态检测与分析

doi:10.11843/j.issn.0366-6964.2014.11.007

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (11): 1784-1792.doi: 10.11843/j.issn.0366-6964.2014.11.007

北京油鸡肌肉和卵巢组织基因组DNA甲基化状态检测与分析

李金龙^1#,唐韶青^2#,赵萌¹,王海潮¹,徐青^1*

(1.北京交通大学生命科学与生物工程研究院，北京 100044； 2.北京市畜牧兽医总站，北京 100029)

收稿日期:2014-03-27 出版日期:2014-11-23 发布日期:2014-11-23
通讯作者: 徐青，副教授，E-mail：qingxu@bjtu.edu.cn
作者简介:李金龙(1988-)，男，满族，河北承德人，硕士生，主要从事分子遗传学研究，E-mail：lijinlong880827@163.com；唐韶青，博士，主要从事动物分子数量遗传学研究，E-mail：tangshaoqingcau@163.com。李金龙和唐韶青为共同第一作者
基金资助:
北京市自然科学基金资助项目(6112018)；中央高校基本科研业务费专项基金资助项目(2012JBE003)；国家自然科学基金青年基金资助项目(31101711)

Detection of DNA Methylation in Whole Genomes of Muscle and Ovary Tissues of Beijing You Chicken

LI Jin-long^1#，TANG Shao-qing^2#，ZHAO Meng¹，WANG Hai-chao¹，XU Qing^1*

(1.Institute of Life Science and Biotechnology，Beijing Jiaotong University,Beijing 100044,China;2.Beijing Animal Husbandry Station,Beijing 100029，China)

Received:2014-03-27 Online:2014-11-23 Published:2014-11-23

摘要/Abstract

摘要：

以17周龄北京油鸡为试验材料，应用甲基化敏感扩增多态性(Methylation sensitive amplified polymorphism，MSAP)方法检测肌肉和卵巢组织的基因组DNA甲基化模式和程度，进而分析肌肉和卵巢组织基因组DNA甲基化的差异。结果表明，应用12对选择性扩增引物共产生4 297条清晰可辨的扩增条带，平均每个个体每对引物组合可获得10～20个条带。在检测的个体中，肌肉组织的甲基化水平为35.86%，卵巢组织为31.50%，2种组织的甲基化水平差异显著(P<0.01)。北京油鸡体重与肌肉组织的甲基化水平存在极显著负相关(P<0.01)，与卵巢组织的甲基化水平的相关性未达到显著水平(P>0.05)。分离鉴定了5个组织特异性的甲基化片段。结果显示，北京油鸡肌肉和卵巢组织的基因组DNA的甲基化状态存在差异，而肌肉组织的甲基化状态对北京油鸡的体重具有重要作用。

Abstract:

The DNA methylation extent and pattern was assessed in genomes in muscle and ovary tissues from the 17-week-old Beijing You chicken using a methylation sensitive amplified polymorphism (MSAP) approach in this study.We analyzed different DNA methylation levels in different tissues.A total of 4 297 clear bands were obtained using 12 pairs of selective amplification primers，about 10-20 bands per sample with a pair of primers.The degree of methylation was approximate 35.86% in muscle and 31.50% in ovary in Beijing You chickens，and a significant difference was found between 2 tissues at the methylation levels (P<0.01).Further analysis indicated that no significant relationship was detected in ovary tissue between the methylation levels and individual weight (P>0.05)，but strongly negative correlation was depicted in muscle tissue (P<0.01).Five tissue-specific MSAP fragments were isolated and verified.Overall results reveal that there is a significant difference at the methylation levels between muscle and ovary tissues，and the methylated state of muscle tissue may play an important role in the body weight of Beijing You chicken.

中图分类号:

S831.2
Q75

李金龙,唐韶青,赵萌,王海潮,徐青. 北京油鸡肌肉和卵巢组织基因组DNA甲基化状态检测与分析[J]. 畜牧兽医学报, 2014, 45(11): 1784-1792.

LI Jin-long，TANG Shao-qing，ZHAO Meng，WANG Hai-chao，XU Qing. Detection of DNA Methylation in Whole Genomes of Muscle and Ovary Tissues of Beijing You Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1784-1792.

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参考文献

［1］RAZIN A，CEDAR H.DNA methylation and gene expression ［J］.Microbiol Rev，1991，55(3)：451-458.
［2］JAENISCH R，BIRD A.Epigenetic regulation of gene expression：how the genome integrates intrinsic and environmental signals ［J］.Nat Genet，2003，33(Suppl)：245-254.
［3］JONES P A.Functions of DNA methylation：islands，start sites，gene bodies and beyond ［J］.Nat Rev Genet，2012，13(7)：484-492.
［4］BORGEL J，GUIBERT S，LI Y F，et al.Targets and dynamics of promoter DNA methylation during early mouse development ［J］.Nat Genet，2010，42(12)：1093-1100.
［5］GOKUL G，KHOSLA S.DNA methylation and cancer ［M］.Epigenetics：Development and Disease，2013：597-625.
［6］唐韶青，张沅，徐青，等.不同动物部分组织基因组甲基化程度的差异分析［J］.农业生物技术学报，2006，14(4)：507-511.
［7］MA J D，LI M Z，ZHOU S L，et al.Methylation-sensitive amplification polymorphism analysis of fat and muscle tissues in pigs ［J］.Genet Mol Res，2012，11(3)：3505-3510.
［8］XU Q，ZHANG Y，SUN D X，et al.Analysis on DNA methylation of various tissues in chicken ［J］.Anim Biotechnol, 2007，18(4)：231-241.
［9］田艳苓.鸡微卫星标记，DNA 甲基化差异和 FSHβ 变异及其与体重和产蛋性状的关系［D］.泰安：山东农业大学，2011.
［10］LU Y L，RONG T Z，CAO M J.Analysis of DNA methylation in different maize tissues ［J］.J Genet Genom，2008，35(1)：41-48.
［11］ZHANG M S，XU C M，WETTSTEIN D V，et al.Tissue-specific differences in cytosine methylation and their association with differential gene expression in sorghum ［J］.Plant Physiol，2011，156(4)：1955-1966.
［12］GEIMAN T M，MUEGGE K.DNA methylation in early development ［J］.Mol Reprod Dev，2010，77(2)：105-113.
［13］YANG C，ZHANG M J，NIU W P，et al.Analysis of DNA methylation in various swine tissues ［J］.PLoS ONE，2011，6(1)：e16229.
［14］GUPTA R，NAGARAJAN A，WAJAPEYEE N.Advances in genome-wide DNA methylation analysis ［J］.Biotechniques，2010，49(4)：iii-xi.
［15］XU M L，LI X Q，KORBAN S S.AFLP-based detection of DNA methylation ［J］.Plant Mol Biol Rep，2000，18(4)：361-368.
［16］GAO F，LUO Y L，LI S T，et al.Comparison of gene expression and genome-wide DNA methylation profiling between phenotypically normal cloned pigs and conventionally bred controls ［J］.PLoS ONE，2011，6(10)：e25901.
［17］DA K，NOWAK J，FLINN B.Potato cytosine methylation and gene expression changes induced by a beneficial bacterial endophyte，Burkholderia phytofirmans strain PsJN ［J］.Plant Physiol Biochem，2012，50(1)：24-34.
［18］LI X Q，XU M L，KORBAN S S.DNA methylation profiles differ between field-and in vitro-grown leaves of apple ［J］.J Plant physiol，2002，159(11)：1229-1234.
［19］DE MONTERA B，BOULANGER L，TAOURIT S，et al.Genetic identity of clones and methods to explore DNA ［J］.Cloning Stem Cells，2004，6(2)：133-139.
［20］YANG W，YU X，LIU B，et al.Parental epigenetic difference in DNA methylation-level may play contrasting roles for different agronomic traits related to yield heterosis in maize ［J］.Afr J Biotechnol，2011，10(46)：9253-9263.
［21］唐韶青.鸡生长与繁殖性状候选基因筛选及标记辅助选择的研究［D］.北京：中国农业大学，2010.
［22］徐青，张沅，孙东晓，等.应用MSAP方法检测鸡不同组织基因组的甲基化状态［J］.遗传，2011，33(6)：620-626.
［23］GRUNAU C，HINDERMANN W，ROSENTHAL A.Large-scale methylation analysis of human genomic DNA reveals tissue-specific differences between the methylation profiles of genes and pseudogenes ［J］.Hum Mol Genet，2000，9(18)：2651-2663.
［24］WAALWIJK C，FLAVELL R A.DNA methylation at a CCGG sequence in the large intron of the rabbit β-globin gene：tissue-specific variations ［J］.Nucleic Acids Res，1978，5(12)：4631-4642.
［25］XIONG L Z，XU C G，SAGHAI MAROOF M A，et al.Patterns of cytosine methylation in an elite rice hybrid and its parental lines，detected by a methylation-sensitive amplification polymorphism technique ［J］.Mol Gen Genet，1999，261(3)：439-446.
［26］PERAZA-ECHEVERRIA S，HERRERA-VALENCIA V A，ANDREW-JAMES K.Detection of DNA methylation changes in micropropagated banana plants using methylation-sensitive amplification polymorphism (MSAP) ［J］.Plant Sci，2001，161(2)：359-367.
［27］RIGGS A D.X inactivation，differentiation，and DNA methylation ［J］.Cytogenet Genome Res，1975，14(1)：9-25.
［28］刘健.DLY 猪不同部位脂肪和肌肉组织基因组甲基化程度的差异［D］.雅安：四川农业大学，2009.
［29］HASHIMSHONY T，ZHANG J M，KESHET I，et al.The role of DNA methylation in setting up chromatin structure during development ［J］.Nat Genet，2003，34(2)：187-192.
［30］ABASHT B，DEKKERS J C，LAMONT S J.Review of quantitative trait loci identified in the chicken ［J］.Poult Sci，2006，85(12)：2079-2096.
［31］UEMOTO Y，SATO S，ODAWARA S，et al.Genetic mapping of quantitative trait loci affecting growth and carcass traits in F2 intercross chickens ［J］.Poult Sci，2009，88(3)：477-482.
［32］PODISI B K，KNOTT S A，BURT D W，et al.Comparative analysis of quantitative trait loci for body weight，growth rate and growth curve parameters from 3 to 72 weeks of age in female chickens of a broiler-layer cross ［J］.BMC Genet，2013，14(1)：22.
［33］蒋曹德，邓昌彦，熊远著.猪个体 DNA 甲基化百分差异与胴体性状的关系［J］.农业生物技术学报，2005，13(2)：179-185.
［34］万亚琴.DNA 甲基化与肉牛杂种优势关系的初步研究［D］.重庆：西南大学，2008.
［35］SONG F，SMITH J F，KIMURA M T，et al.Association of tissue-specific differentially methylated regions (TDMs) with differential gene expression ［J］.Proc Natl Acad Sci USA，2005，102(9)：3336-3341.

北京油鸡肌肉和卵巢组织基因组DNA甲基化状态检测与分析

Detection of DNA Methylation in Whole Genomes of Muscle and Ovary Tissues of Beijing You Chicken

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